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Square beams for optimal tiling in transmission electron microscopy.

Eugene Y D ChuaLambertus M AlinkMykhailo KopylovJake D JohnstonFabian EisensteinAlex de Marco
Published in: Nature methods (2024)
Imaging large fields of view at a high magnification requires tiling. Transmission electron microscopes typically have round beam profiles; therefore, tiling across a large area is either imperfect or results in uneven exposures, a problem for dose-sensitive samples. Here, we introduce a square electron beam that can easily be retrofitted in existing microscopes, and demonstrate its application, showing that it can tile nearly perfectly and deliver cryo-electron microscopy imaging with a resolution comparable to conventional set-ups.
Keyphrases
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