Single-Molecule Rotation for EGFR Conformational Dynamics in Live Cells.
Youngchan ParkSangwon ShinHyeonggyu JinJiseong ParkYeonki HongJaemin ChoiByunghyuck JungHyunjoon SongDaeha SeoPublished in: Journal of the American Chemical Society (2018)
Monitoring the dynamics of proteins in live cells on appropriate spatiotemporal scales may provide key information regarding long-standing questions in molecular and cellular regulatory mechanisms. However, tools capable of imaging the conformational changes over time have been elusive. Here, we present a single-molecule stroboscopic imaging probes by developing gyroscopic plasmonic nanoparticles, allowing for replication of protein-protein interactions and the conformational dynamics based on rotational and lateral velocities. This study fundamentally monitors the rotational motion of a membrane protein, epidermal growth factor receptor (EGFR), to decipher undiscovered structural dynamics in live cells without any molecular perturbations. This method offers a strategy to visualize assemblies and conformational changes, and provides unique insights into the mechanism underlying the molecular dynamics for receptors.
Keyphrases
- single molecule
- epidermal growth factor receptor
- molecular dynamics
- induced apoptosis
- living cells
- atomic force microscopy
- cell cycle arrest
- tyrosine kinase
- small cell lung cancer
- high resolution
- healthcare
- cell death
- signaling pathway
- oxidative stress
- density functional theory
- transcription factor
- health information
- pi k akt
- social media
- cell proliferation