Operational utility of the reverse-transcription recombinase polymerase amplification for detection of dengue virus.
Kim-Kee TanNoor Syahida AzizanChe Norainon YaacobNurul Asma Anati Che Mat SeriNur Izyan SamsudinBoon-Teong TeohSing-Sin SamSazaly Abu BakarPublished in: BMC infectious diseases (2018)
The dengue RT-RPA assay can be successfully performed by simply following the provided written instructions. Deviations from the written protocols did not adversely affect the outcome of the assay. These suggest that the RT-RPA assay is indeed a simple, robust and efficient laboratory method for detection of dengue virus. Furthermore, high new user acceptance of the RT-RPA assay suggests that this assay could be successfully deployed into new laboratories where RT-RPA was not previously performed.