Impact of long-term storage of plasma and cell-free DNA on measured DNA quantity and fetal fraction.
Frederik Banch ClausenAngela N BarrettHenna V AdvaniMahesh ChoolaniMorten Hanefeld DziegielPublished in: Vox sanguinis (2020)
Fetal and total cell-free DNA is readily detectable in plasma after long-term storage at -25°C. However, substantial variation in measured DNA quantities and fetal fraction means caution may be required when using stored plasma and extracted cfDNA for test development or validation purposes.