Hybrid de novo genome assembly and centromere characterization of the gray mouse lemur (Microcebus murinus).

Peter A LarsenR Alan HarrisYue LiuShwetha C MuraliC Ryan CampbellAdam D BrownBeth A SullivanJennifer SheltonSusan J BrownMuthuswamy RaveendranOlga DudchenkoIdo MacholNeva C DurandMuhammad S ShamimErez Lieberman AidenDonna M MuznyRichard A GibbsAnne D YoderJeffrey RogersKim C Worley

Published in: BMC biology (2017)

We describe a new assembly of the gray mouse lemur (Microcebus murinus) genome with chromosome-scale scaffolds produced using a hybrid bioinformatic and sequencing approach. The approach is cost effective and produces superior results based on metrics of contiguity and completeness. Our results show that emerging genomic technologies can be used in combination to characterize centromeres of non-model species and to produce accurate de novo chromosome-scale genome assemblies of complex mammalian genomes.

Keyphrases

copy number
genome wide
single cell
dna methylation
gene expression
mass spectrometry
tissue engineering
genetic diversity