Epigenome-wide effects of vitamin D on asthma bronchial epithelial cells.
Nadia BoutaouiSandeep PuranikRong ZhangTing WangDaniel H HuiJohn BrehmErick FornoWei ChenJuan C CeledónPublished in: Epigenetics (2019)
Vitamin D is a nutrient and a hormone with multiple effects on immune regulation and respiratory viral infections, which can worsen asthma and lead to severe asthma exacerbations. We set up a complete experimental and analytical pipeline for ATAC-Seq and RNA-Seq to study genome-wide epigenetic changes in human bronchial epithelial cells of asthmatic subjects, following treatment of these cells with calcitriol (vitamin D3) and Poly (I:C)(a viral analogue). This approach led to the identification of biologically plausible candidate genes for viral infections and asthma, such as DUSP10 and SLC44A1.
Keyphrases
- rna seq
- chronic obstructive pulmonary disease
- lung function
- genome wide
- dna methylation
- single cell
- sars cov
- cystic fibrosis
- allergic rhinitis
- induced apoptosis
- endothelial cells
- air pollution
- induced pluripotent stem cells
- endoplasmic reticulum stress
- copy number
- mass spectrometry
- oxidative stress
- combination therapy
- liquid chromatography