Targeted versus Shotgun Metagenomic Sequencing-Based Detection of Microorganisms in Sonicate Fluid for Periprosthetic Joint Infection Diagnosis.
Hyo Lim HongLaure FlurinMatthew J ThoendelMatthew J WolfMatthew P AbdelKerryl E Greenwood-QuaintanceRobin PatelPublished in: Clinical infectious diseases : an official publication of the Infectious Diseases Society of America (2022)
16S rRNA gene-based tNGS is a potential diagnostic tool for PJI pathogen identification in sonicate fluid from failed THAs and TKAs in culture-negative cases, with similar performance characteristics to sNGS.
Keyphrases
- genome wide
- single cell
- cancer therapy
- total hip
- loop mediated isothermal amplification
- candida albicans
- antibiotic resistance genes
- label free
- gene expression
- total hip arthroplasty
- human health
- drug delivery
- bioinformatics analysis
- dna methylation
- microbial community
- total knee arthroplasty
- wastewater treatment
- genome wide analysis