Single-cell analysis reveals that cryptic prophage protease LfgB protects Escherichia coli during oxidative stress by cleaving antitoxin MqsA.
Laura Fernandez-GarciaXinyu GaoJoy KirigoSooyeon SongMichael E BattistiRodolfo Garcia-ContrerasMaria TomasXiaoxue WangXiaoxue WangThomas K WoodPublished in: Microbiology spectrum (2024)
The roles of toxin/antitoxin systems in cell physiology are few and include phage inhibition and stabilization of genetic elements; yet, to date, there are no single-transcriptome studies for toxin/antitoxin systems and few insights for prokaryotes from this novel technique. Therefore, our results with this technique are important since we discover and characterize a cryptic prophage protease that is regulated by the MqsR/MqsA toxin/antitoxin system in order to regulate the host response to oxidative stress.
Keyphrases
- escherichia coli
- single cell
- oxidative stress
- rna seq
- dna damage
- genome wide
- high throughput
- biofilm formation
- ischemia reperfusion injury
- klebsiella pneumoniae
- induced apoptosis
- diabetic rats
- pseudomonas aeruginosa
- gene expression
- stem cells
- copy number
- case control
- heat shock
- heat shock protein
- endoplasmic reticulum stress