INTRODUCING THE NO SIGNIFICANT EFFECT CONCENTRATION (NSEC).

The No Effect Concentration (NEC) is the preferred threshold metric for single species toxicity tests applied to derive safe concentration thresholds for contaminants in the environment for use in Species Sensitivity Distributions (SSDs). However, the NEC is only suitable when concentration-response (C-R) data exhibit a threshold response. Here we describe an alternative toxicity estimate, the no-significant-effect-concentration (NSEC) which is better suited to C-R data for which the response is a monotonically decreasing function of concentration and no threshold effects are evident. We use a flexible, three-parameter sigmoidal function to describe the C-R relationship and detail both Bayesian and Frequentist approaches to estimation and inference for the NSEC. While the NSEC is conceptually linked to the traditional no-observed-effect-concentration (NOEC), it is a substantial improvement over the NOEC because it decouples the estimate from being directly dependent on the placement of treatment concentrations as well as admitting statements of precision of the resulting toxicity estimate. This article is protected by copyright. All rights reserved. Environ Toxicol Chem 2023;00:0-0. © 2023 SETAC.