Bottom-up/cross-linking mass spectrometry via simplified sample processing on anion-exchange solid-phase extraction spin column.
Ayako TakemoriYusuke KawashimaNobuaki TakemoriPublished in: Chemical communications (Cambridge, England) (2022)
We introduce a simple single-column protein digestion method for low-microgram-level samples containing sodium dodecyl sulfate and Coomassie dye that can be completed within a few hours.
Keyphrases
- solid phase extraction
- liquid chromatography
- high performance liquid chromatography
- mass spectrometry
- tandem mass spectrometry
- gas chromatography
- liquid chromatography tandem mass spectrometry
- molecularly imprinted
- high resolution mass spectrometry
- simultaneous determination
- gas chromatography mass spectrometry
- ultra high performance liquid chromatography
- ionic liquid
- density functional theory
- protein protein
- high resolution
- single molecule
- highly efficient
- binding protein
- anaerobic digestion
- transition metal