Detection of CAR-T19 cells in peripheral blood and cerebrospinal fluid: An assay applicable to routine diagnostic laboratories.
Ulrika JohanssonKathleen GallagherVictoria BurgoyneMarcela V MausKeagan S CaseyGabrielle G BriniMatthew J FrigaultJennifer Y YamNikesh ChavdaCaroline BesleySanne LugthartPublished in: Cytometry. Part B, Clinical cytometry (2021)
The assay enabled routine monitoring of CAR-T19 in blood and CSF samples. Despite profound cytopenia in many lymphoma patients, results were obtained regularly from only 4 ml of blood. The assay can be adapted easily to characterize the memory and exhaustion status of CAR-T19 and native T-cells. Importantly, it does not rely on CAR construct specificity; thus, it can be used to detect any CD19-targeted CAR cell. Finally, our validation process can serve as a blueprint for other fluorochrome proteins used to detect CAR cells.
Keyphrases
- induced apoptosis
- cerebrospinal fluid
- high throughput
- peripheral blood
- cell cycle arrest
- end stage renal disease
- newly diagnosed
- chronic kidney disease
- single cell
- prognostic factors
- working memory
- diffuse large b cell lymphoma
- drug delivery
- endoplasmic reticulum stress
- cancer therapy
- intellectual disability
- stem cells
- cell death
- signaling pathway
- oxidative stress
- peritoneal dialysis
- mesenchymal stem cells
- quantum dots
- real time pcr
- patient reported