Evaluation of Lymphocyte Response to the Induced Oxidative Stress in a Cohort of Ageing Subjects, including Semisupercentenarians and Their Offspring.
Federico SizzanoSebastiano CollinoOrnella CominettiDaniela MontiPaolo GaragnaniRita OstanChiara PirazziniMaria Giulia BacaliniDaniela MariGiuseppe PassarinoClaudio FranceschiAlessio PaliniPublished in: Mediators of inflammation (2018)
The production of reactive oxygen species (ROS) may promote immunosenescence if not counterbalanced by the antioxidant systems. Cell membranes, proteins, and nucleic acids become the target of ROS and progressively lose their structure and functions. This process could lead to an impairment of the immune response. However, little is known about the capability of the immune cells of elderly individuals to dynamically counteract the oxidative stress. Here, the response of the main lymphocyte subsets to the induced oxidative stress in semisupercentenarians (CENT), their offspring (OFF), elderly controls (CTRL), and young individuals (YO) was analyzed using flow cytometry. The results showed that the ratio of the ROS levels between the induced and noninduced (I/NI) oxidative stress conditions was higher in CTRL and OFF than in CENT and YO, in almost all T, B, and NK subsets. Moreover, the ratio of reduced glutathione levels between I/NI conditions was higher in OFF and CENT compared to the other groups in almost all the subsets. Finally, we observed significant correlations between the response to the induced oxidative stress and the degree of methylation in specific genes on the oxidative stress pathway. Globally, these data suggest that the capability to buffer dynamic changes in the oxidative environment could be a hallmark of longevity in humans.
Keyphrases
- oxidative stress
- reactive oxygen species
- dna damage
- peripheral blood
- diabetic rats
- flow cytometry
- hydrogen peroxide
- immune response
- middle aged
- cell death
- ischemia reperfusion injury
- induced apoptosis
- high fat diet
- community dwelling
- dna methylation
- electronic health record
- single cell
- adipose tissue
- skeletal muscle
- stem cells
- endothelial cells
- metabolic syndrome
- toll like receptor
- inflammatory response
- deep learning
- metal organic framework
- endoplasmic reticulum stress