Priming mesenchymal stromal cells with neurotrophic factors boosts the neuro-regenerative potential of their secretome.
Prajakta TeliAditya NachanekarVaijayanti P KaleAnuradha VaidyaPublished in: Regenerative medicine (2023)
Aim: To explore the neuroprotective potential of the secretome (conditioned medium, CM) derived from neurotrophic factors-primed mesenchymal stromal cells (MSCs; primed CM) using an endoplasmic reticulum (ER) stress-induced in vitro model system. Methods: Establishment of ER-stressed in vitro model, immunofluorescence microscopy, real-time PCR, western blot. Results: Exposure of ER-stressed Neuro-2a cells to the primed-CM significantly restored the neurite outgrowth parameters and improved the expression of neuronal markers like Tubb3 and Map2a in them compared with the naive CM. Primed CM also suppressed the induction of apoptotic markers Bax and Sirt1 , inflammatory markers Cox2 and NF-κB, and stress kinases such as p38 and SAPK/JNK in the stress-induced cells. Conclusion: The secretome from primed MSCs significantly restored ER stress-induced loss of neuro-regenesis.
Keyphrases
- stress induced
- endoplasmic reticulum
- induced apoptosis
- signaling pathway
- mesenchymal stem cells
- cell cycle arrest
- oxidative stress
- cell death
- endoplasmic reticulum stress
- estrogen receptor
- bone marrow
- real time pcr
- stem cells
- breast cancer cells
- pi k akt
- umbilical cord
- high resolution
- risk assessment
- lps induced
- south africa
- human health
- cerebral ischemia
- subarachnoid hemorrhage
- high throughput
- nuclear factor
- high speed
- mass spectrometry
- inflammatory response
- ischemia reperfusion injury
- long non coding rna
- heat stress