Ticagrelor alleviates high-carbohydrate intake induced altered electrical activity of ventricular cardiomyocytes by regulating sarcoplasmic reticulum-mitochondria miscommunication.
Yusuf OlgarAysegul DurakSinan DegirmenciErkan TuncayDeniz BillurSemir OzdemirBelma TuranPublished in: Molecular and cellular biochemistry (2021)
Metabolic syndrome (MetS) is associated with additional cardiovascular risk in mammalians while there are relationships between hyperglycemia-associated cardiovascular dysfunction and increased platelet P2Y12 receptor activation. Although P2Y12 receptor antagonist ticagrelor (Tica) plays roles in reduction of cardiovascular events, its beneficial mechanism remains poorly understood. Therefore, we aimed to clarify whether Tica can exert a direct protective effect in ventricular cardiomyocytes from high-carbohydrate diet-induced MetS rats, at least, through affecting sarcoplasmic reticulum (SR)-mitochondria (Mit) miscommunication. Tica treatment of MetS rats (150 mg/kg/day for 15 days) significantly reversed the altered parameters of action potentials by reversing sarcolemmal ionic currents carried by voltage-dependent Na+ and K+ channels, and Na+/Ca2+-exchanger in the cells, expressed P2Y12 receptors. The increased basal-cytosolic Ca2+ level and depressed SR Ca2+ load were also reversed in Tica-treated cells, at most, though recoveries in the phosphorylation levels of ryanodine receptors and phospholamban. Moreover, there were marked recoveries in Mit structure and function (including increases in both autophagosomes and fragmentations) together with recoveries in Mit proteins and the factors associated with Ca2+ transfer between SR-Mit. There were further significant recoveries in markers of both ER stress and oxidative stress. Taken into consideration the Tica-induced prevention of ER stress and mitochondrial dysfunction, our data provided an important document on the pleiotropic effects of Tica in the electrical activity of the cardiomyocytes from MetS rats. This protective effect seems through recoveries in SR-Mit miscommunication besides modulation of different sarcolemmal ion-channel activities, independent of P2Y12 receptor antagonism.
Keyphrases
- high glucose
- induced apoptosis
- oxidative stress
- diabetic rats
- cardiovascular events
- cell cycle arrest
- metabolic syndrome
- acute coronary syndrome
- protein kinase
- cell death
- endothelial cells
- percutaneous coronary intervention
- heart failure
- coronary artery disease
- endoplasmic reticulum stress
- left ventricular
- signaling pathway
- dna damage
- drug induced
- electronic health record
- antiplatelet therapy
- body mass index
- ischemia reperfusion injury
- endoplasmic reticulum
- combination therapy
- big data
- pi k akt
- cell proliferation
- insulin resistance
- catheter ablation
- heat stress
- solid state
- electron transfer
- binding protein