Downregulation of UBE2T can enhance the radiosensitivity of osteosarcoma in vitro and in vivo.
Lin ShenKai ZhaoHan LiBin NingWenzhao WangRonghan LiuYining ZhangAijun ZhangPublished in: Epigenomics (2019)
Aim: To investigate the effect of UBE2T gene on radiotherapy for osteosarcoma. Materials & methods: Gene Expression Omnibus database, RT-qPCR and immunohistochemical analysis were performed. Cell proliferation and cell cycle experiments were conducted after knockdown of UBE2T. Cell scratch, reactive oxygen species production and apoptosis experiments were conducted after the combination of radiotherapy and UBE2T silencing. Then the xenograft mode was further conducted. Results: UBE2T was highly expressed in human osteosarcoma. Suppression of UBE2T inhibited osteosarcoma cell proliferation and induced cell cycle arrest at the G2/M phase. Downregulation of UBE2T combined with radiation can substantially inhibit clonal formation and migration, and promote apoptosis of osteosarcoma cells in vitro and in vivo. Conclusion: UBE2T downregulation can enhance the radiosensitivity of osteosarcoma in vitro and in vivo.
Keyphrases
- cell cycle arrest
- cell proliferation
- cell cycle
- pi k akt
- cell death
- signaling pathway
- gene expression
- early stage
- reactive oxygen species
- endoplasmic reticulum stress
- oxidative stress
- induced apoptosis
- endothelial cells
- dna methylation
- radiation therapy
- squamous cell carcinoma
- emergency department
- genome wide
- diabetic rats
- electronic health record