Evaluation of ERIC-PCR and MALDI-TOF as typing tools for multidrug resistant Klebsiella pneumoniae clinical isolates from a tertiary care center in India.
Jyoti KunduShubhangi KansalShivali RathoreMeenakshi KaundalArchana AngrupManisha BiswalKamini WaliaPallab RayPublished in: PloS one (2022)
The study presents ERIC-PCR as more robust and better discriminatory typing tool in comparison to MALDI-TOF for clonal relatedness in multidrug resistant K. pneumoniae clinical isolates. Isolates were typed into 40 ERIC types, and six groups by MALDI-TOF-MS. PCR-based analysis revealed that all the strains harbored two or more ESBL and carbapenemase genes. None of the isolates revealed the presence of the plasmid mediated mcr-1 gene for colistin resistance.
Keyphrases
- klebsiella pneumoniae
- multidrug resistant
- mass spectrometry
- escherichia coli
- genetic diversity
- acinetobacter baumannii
- gram negative
- drug resistant
- tertiary care
- genome wide
- single cell
- real time pcr
- ms ms
- genome wide identification
- crispr cas
- copy number
- gene expression
- dna methylation
- cystic fibrosis
- pseudomonas aeruginosa
- bioinformatics analysis