Influence of heat stress on reference genes stability in heart and liver of two chickens genotypes.
Juliana Gracielle Gonzaga GromboniHaniel Cedraz de OliveiraDaniele Botelho Diniz MarquesAntônio Amândio Pinto Garcia JuniorRonaldo Vasconcelos Farias FilhoCaio Fernando GromboniTeillor Machado SouzaAmauri Arias WenceslauPublished in: PloS one (2020)
According to the RankAggreg tool classification based on the three different algorithms (BestKeeper, geNorm and NormFinder), the most stable genes were RPL5, EEF1 and LDHA for heart tissue and RPL5, EEF1 and ACTB for liver tissue of chickens subjected to comfort and acute heat stress environmental conditions. However, the best reference genes may vary depending on the experimental conditions of each study, such as different breeds, environmental stressors, and tissues analyzed. Therefore, the need to perform priori studies to assay the best reference genes at the outset of each study is emphasized.