Highly Flexible Single-Unit Resolution All Printed Neural Interface on a Bioresorbable Backbone.

Neural interfaces are the parts of the neural prosthesis that are in contact with the target tissue. The mechanical, chemical, and electrical properties of these interfaces can be a major determinant of the life of the implant and the neural tissue for chronic and even acute integrations. In this work, we developed a fully inkjet-printed, flexible neural interface on a bioresorbable backbone capable of recording high-fidelity neural activity. We utilized room temperature fabrication processes that overcome the limitations of semiconductor fabrication techniques for processing low-melting point polymers while maintaining high spatial and single-cell recording resolution. The ∼8 μm-thick devices in this study were fabricated onto two flexible polymers: (a) polyimide (PI), a biocompatible polymer commonly used for neural interfaces, and (b) polycaprolactone (PCL), a bioresorbable polyester with outstanding mechanical properties. Electrodes for neural recording were built at 30, 50, 75, and 100 μm diameter using silver nanoparticles/(3,4-ethylenedioxytiophene)-poly(styrenesulfonate) (AgNPs/PEDOT:PSS), which through our process achieved the lowest impedance reported in the literature reaching ∼200 Ω at 1 kHz for a 50 μm electrode diameter. We further enhanced the electrochemical performance of AgNPs/PEDOT:PSS by an order of magnitude by incorporating exfoliated graphene into the electrodes. The biocompatibility of the fabricated devices and their ability to record single-unit activity were confirmed by in vitro tests on both rat PC12 cells and isolated neural rat retina, respectively.