NanoSOSG: A Nanostructured Fluorescent Probe for the Detection of Intracellular Singlet Oxygen.
Rubén Ruiz-GonzálezRoger Bresolí-ObachÒscar GulíasMontserrat AgutHuguette SavoieRoss W BoyleSanti NonellFrancesca GiuntiniPublished in: Angewandte Chemie (International ed. in English) (2017)
A biocompatible fluorescent nanoprobe for singlet oxygen (1 O2 ) detection in biological systems was designed, synthesized, and characterized, that circumvents many of the limitations of the molecular probe Singlet Oxygen Sensor Green® (SOSG). This widely used commercial singlet oxygen probe was covalently linked to a polyacrylamide nanoparticle core using different architectures to optimize the response to 1 O2 . In contrast to its molecular counterpart, the optimum SOSG-based nanoprobe, which we call NanoSOSG, is readily internalized by E. coli cells and does not interact with bovine serum albumin. Furthermore, the spectral characteristics do not change inside cells, and the probe responds to intracellularly generated 1 O2 with an increase in fluorescence.
Keyphrases
- living cells
- fluorescent probe
- single molecule
- induced apoptosis
- energy transfer
- quantum dots
- cell cycle arrest
- loop mediated isothermal amplification
- escherichia coli
- label free
- magnetic resonance
- endoplasmic reticulum stress
- oxidative stress
- cell death
- magnetic resonance imaging
- optical coherence tomography
- real time pcr
- cell proliferation
- drug release
- signaling pathway
- reactive oxygen species
- sensitive detection
- contrast enhanced