Protein identification in imaging mass spectrometry through spatially targeted liquid micro-extractions.
Daniel J RyanDavid NeiBoone M PrenticeKristie L RoseRichard Micheal CaprioliJeffrey M SpragginsPublished in: Rapid communications in mass spectrometry : RCM (2018)
Robotic liquid surface extractions can be used to interrogate discrete regions of tissue to provide protein identifications with high throughput, accuracy, and robustness. The direct coupling of tissue surface extractions and LC offers a new and effective approach to provide spatial proteomics data in an imaging experiment.
Keyphrases
- mass spectrometry
- high resolution
- high throughput
- liquid chromatography
- protein protein
- ionic liquid
- amino acid
- binding protein
- minimally invasive
- high performance liquid chromatography
- electronic health record
- gas chromatography
- single cell
- big data
- small molecule
- drug delivery
- fluorescence imaging
- bioinformatics analysis
- ms ms
- deep learning