A Fluorescence-Based High-Throughput Screening Method for Olefin Metathesis Using a Ratiometric Fluorescent Probe.

(Z)-1,8-Di(pyren-1-yl)oct-4-ene (1) was prepared as a probe for olefin metathesis. The conversions of substrate by olefin metathesis under various conditions were calculated using the ratiometric fluorescence intensity change of 1. The conversions calculated by 1 and gas chromatography were consistent. These results show that conversions of olefin metathesis can be simply obtained from the fluorescence change of 1 and this method can be applied to the high-throughput screening (HTS) method for various olefin metathesis.