9-cis-β-Apo-10'-carotenal is the precursor of strigolactones in planta.

C-labeled 9-cis-β-apo-10'-carotenal and analyzed their SLs by LC-MS. Our results show that 9-cis-β-apo-10'-carotenal is the SL precursor in planta and reveal, for the first time, the application of labeled long-chain apocarotenoids as a promising approach to investigate apocarotenoid metabolism and the genesis of carotenoid-derived growth regulators and signaling molecules.