Evaluation of the performance of an in-house duplex PCR assay targeting the IS6110 and rpoB genes for tuberculosis diagnosis in Cameroon.

Henry Dilonga MerikiNdze Henry WungKukwah Anthony TufonNyeke James TonyIrene Ane-AnyangweFidelis Cho-Ngwa

Published in: BMC infectious diseases (2020)

IS1610 and rpoB duplex PCR using relatively cheap decontamination and DNA extraction methods in addition to simple serial dilutions to resolve PCR inhibition shows high sensitivity in the diagnosis of paucibacillary tuberculosis.

Keyphrases

mycobacterium tuberculosis
real time pcr
pulmonary tuberculosis
hiv aids
high throughput
genome wide
dna methylation
single cell
bioinformatics analysis
electronic health record