An antiviral role for TRIM14 in Ebola virus infection.

Ebola virus (EBOV) is a highly pathogenic virus that encodes seven multifunctional structural proteins. Multiple host factors have been reported to interact with the EBOV proteins. Here, we found that Tripartite motif-containing 14 (TRIM14), an interferon-stimulated gene that mediates cellular signaling pathways associated with type I interferon and inflammatory cytokine production, interacts with EBOV nucleoprotein (NP) to enhance IFN-β and NF-κB promotor activation. Moreover, TRIM14 overexpression reduced viral replication in an infectious but biologically contained EBOVΔVP30 system by approximately 10-fold without affecting viral protein expression. Furthermore, TRM14-deficient mice were more susceptible to mouse-adapted EBOV infection than wild-type mice. Our data suggest that TRIM14 is a host factor with anti-EBOV activity that limits EBOV pathogenesis.