A facile Immunoregulatory Constructional Design by Proanthocyanidin Optimizing Directional Chitosan Microchannel.
Xing LiYuxiang WangChengkun ZhaoLei TongPeilei WangJie LiangQing JiangYujiang FanXingdong ZhangYong SunPublished in: Small (Weinheim an der Bergstrasse, Germany) (2024)
Improving the interconnected structure and bioregulatory function of natural chitosan is beneficial for optimizing its performance in bone regeneration. Here, a facile immunoregulatory constructional design is proposed for developing instructive chitosan by directional freezing and alkaline salting out. The molecular dynamics simulation confirmed the assembly kinetics and structural features of various polyphenols and chitosan molecules. Along with the in vitro anti-inflammatory, antioxidative, promoting bone mesenchymal stem cell (BMSC) adhesion and proliferation performance, proanthocyanidin optimizing chitosan (ChiO) scaffold presented an optimal immunoregulatory structure with the directional microchannel. Transcriptome analysis in vitro further revealed the cytoskeleton- and immune-regulation effect of ChiO are the key mechanism of action on BMSC. The rabbit cranial defect model (Φ = 10 mm) after 12 weeks of implantation confirmed the significantly enhanced bone reconstitution. This facile immunoregulatory directional microchannel design provides effective guidance for developing inducible chitosan scaffolds.
Keyphrases
- drug delivery
- bone regeneration
- wound healing
- hyaluronic acid
- molecular dynamics simulations
- anti inflammatory
- quantum dots
- mesenchymal stem cells
- bone mineral density
- bone marrow
- highly efficient
- reduced graphene oxide
- escherichia coli
- body composition
- gold nanoparticles
- metal organic framework
- preterm birth
- cell migration