Development of in vitro model of exosome transport in microfluidic gut-brain axis-on-a-chip.
Gwang Myeong SeoHongki LeeYeon Jae KangDonghyun KimJong Hwan SungPublished in: Lab on a chip (2024)
The gut communicates with the brain in a variety of ways known as the gut-brain axis (GBA), which is known to affect neurophysiological functions as well as neuronal disorders. Exosomes capable of passing through the blood-brain-barrier (BBB) have received attention as a mediator of gut-brain signaling and drug delivery vehicles. In conventional well plate-based experiments, it is difficult to observe the exosome movement in real time. Here, we developed a microfluidic-based GBA chip for co-culturing gut epithelial cells and neuronal cells and simultaneously observing exosome transport. The GBA-chip is aimed to mimic the in vivo situation of convective flow in blood vessels and convective and diffusive transport in the tissue interstitium. Here, fluorescence-labeled exosome was produced by transfection of HEK-293T cells with CD63-GFP plasmid. We observed in real time the secretion of CD63-GFP-exosomes by the transfected HEK-293T cells in the chip, and transport of the exosomes to neuronal cells and analyzed the dynamics of GFP-exosome movement. Our model is expected to enhance understanding of the roles of exosome in GBA.
Keyphrases
- circulating tumor cells
- high throughput
- cerebral ischemia
- resting state
- induced apoptosis
- white matter
- mesenchymal stem cells
- drug delivery
- stem cells
- functional connectivity
- cell cycle arrest
- blood brain barrier
- single cell
- escherichia coli
- working memory
- subarachnoid hemorrhage
- computed tomography
- endoplasmic reticulum stress
- pet imaging
- brain injury
- crispr cas
- oxidative stress
- bone marrow
- quantum dots
- positron emission tomography