Effects of Low-Intensity and Long-Term Aerobic Exercise on the Psoas Muscle of mdx Mice: An Experimental Model of Duchenne Muscular Dystrophy.
Emilly SigoliRosangela Aline AntãoMaria Paula GuerreiroTatiana Oliveira Passos de AraújoPatty Karina Dos SantosDaiane Leite Da RozaDilson Etcheverry RassierAnabelle Silva CornachionePublished in: International journal of molecular sciences (2022)
Duchenne muscular dystrophy (DMD) is a muscle disease characterized by the absence of the protein dystrophin, which causes a loss of sarcolemma integrity, determining recurrent muscle injuries, decrease in muscle function, and progressive degeneration. Currently, there is a need for therapeutic treatments to improve the quality of life of DMD patients. Here, we investigated the effects of a low-intensity aerobic training (37 sessions) on satellite cells, peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1α protein (PGC-1α), and different types of fibers of the psoas muscle from mdx mice (DMD experimental model). Wildtype and mdx mice were randomly divided into sedentary and trained groups ( n = 24). Trained animals were subjected to 37 sessions of low-intensity running on a motorized treadmill. Subsequently, the psoas muscle was excised and analyzed by immunofluorescence for dystrophin, satellite cells, myosin heavy chain (MHC), and PGC-1α content. The minimal Feret's diameters of the fibers were measured, and light microscopy was applied to observe general morphological features of the muscles. The training (37 sessions) improved morphological features in muscles from mdx mice and caused an increase in the number of quiescent/activated satellite cells. It also increased the content of PGC-1α in the mdx group. We concluded that low-intensity aerobic exercise (37 sessions) was able to reverse deleterious changes determined by DMD.
Keyphrases
- duchenne muscular dystrophy
- skeletal muscle
- induced apoptosis
- high fat diet induced
- muscular dystrophy
- cell cycle arrest
- insulin resistance
- end stage renal disease
- endoplasmic reticulum stress
- oxidative stress
- chronic kidney disease
- binding protein
- multiple sclerosis
- signaling pathway
- prognostic factors
- peritoneal dialysis
- type diabetes
- metabolic syndrome
- cell proliferation
- high intensity
- high resolution
- wild type
- single molecule
- pi k akt
- single cell
- adipose tissue