A ruthenium-platinum metal complex that binds to sarcin ricin loop RNA and lowers mRNA expression.

Swapan S JainCraig M AndersonIden A SapseSilvie H LundgrenAbigail K FreerHang HoangKyan JainMadeleine Breshears

Published in: Chemical communications (Cambridge, England) (2018)

IT127 is a dinuclear transition metal complex that contains a Pt(ii) and a Ru(iii) metal center. We have shown that IT127 is significantly more effective in binding the 29-base sarcin ricin loop (SRL) RNA in comparison to Cisplatin, a hallmark anticancer agent. Binding site analysis shows that IT127 prefers purine bases and the GAGA tetraloop region of SRL RNA. Our results with a dihydrofolate reductase (DHFR) model system reveal that IT127 binding to mRNA reduces translation of DHFR enzyme and that the Ru(iii) and Pt(ii) centers in IT127 appear to work in a synergistic manner.

Keyphrases

transition metal
nucleic acid
genome wide
single cell
gene expression
energy transfer
dna methylation