Design strategy for an analyte-compensated fluorescent probe to reduce its toxicity.
Jinlong ZhangShuai MuWeilin WangHuipeng SunShuangqin LiXuezhao ShiYunbo LiuXiaoyan LiuHaixia ZhangPublished in: Chemical communications (Cambridge, England) (2022)
During biological detection, the toxicity caused by probes to living organisms is neglected. In this study, an analyte-compensated fluorescent probe (NP-SN 3 ) was constructed for the detection of H 2 S. Through experiments with HepG2 cells and zebrafish embryos and larvae, the NP-SN 3 probe showed no significant difference in imaging performance compared with the traditional probe (NP-N 3 ) but exhibited lower detection-induced toxicity in the imaging of liver fibrosis in activated HSC-T6 cells. During the development of zebrafish embryos and continuous administration in rats, NP-SN 3 showed a lower death rate, higher hatchability and lower malformation in zebrafish embryos and milder pathological symptoms in stained rat tissues.
Keyphrases
- living cells
- fluorescent probe
- oxidative stress
- liver fibrosis
- loop mediated isothermal amplification
- high resolution
- single molecule
- real time pcr
- label free
- diabetic rats
- gene expression
- small molecule
- wastewater treatment
- fluorescence imaging
- endothelial cells
- high glucose
- zika virus
- mass spectrometry
- aedes aegypti