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Coelenterazine Analogue with Human Serum Albumin-Specific Bioluminescence.

Ryo NishiharaKazuki NiwaTatsunosuke TomitaRyoji Kurita
Published in: Bioconjugate chemistry (2020)
A synthetic luciferin comprising an imidazopyrazinone core, named HuLumino1, was designed to generate specific bioluminescence with human serum albumin (HSA) in real serum samples. HuLumino1 was developed by attaching a methoxy-terminated alkyl chain to C-6 of coelenterazine and by eliminating a benzyl group at C-8. HSA levels were quantified within 5% error margins of an enzyme-linked immunosorbent assay without the need for any sample pretreatments because of the high specificity of HuLumino1.
Keyphrases
  • human serum albumin
  • energy transfer
  • high throughput
  • quantum dots