Cytotoxic, Antidiabetic, and Antioxidant Study of Biogenically Improvised Elsholtzia blanda and Chitosan-Assisted Zinc Oxide Nanoparticles.

In the present study, we have improvised a biogenic method to fabricate zinc oxide nanoparticles (ZnO NPs) using chitosan and an aqueous extract of the leaves of Elsholtzia blanda . Characterization of the fabricated products was carried out with the help of ultraviolet-visible, Fourier transform infrared, X-ray diffraction, field emission scanning electron microscopy, high-resolution transmission electron microscopy, selected area electron diffraction, and energy-dispersive X-ray analyses. The size of the improvised ZnO NP measured between 20 and 70 nm and had a spherical and hexagonal shape. The ZnO NPs proved to be highly effective in the antidiabetic test as the sample showed the highest percentage of enzyme inhibition at 74% ± 3.7, while in the antioxidant test, 78% was the maximum percentage of 2,2-diphenyl-1-picrylhydrazyl hydrate scavenging activity. The cytotoxic effect was investigated against the human osteosarcoma (MG-63) cell line, and the IC 50 value was 62.61 μg/mL. Photocatalytic efficiency was studied by the degradation of Congo red where 91% of dye degradation was observed. From the various analyses, it can be concluded that the as-synthesized NPs may be suitable for various biomedical applications as well as for environmental remediation.