Objective The NUDT15 variants impact thiopurine dose selection in acute lymphoblastic leukemia patients. The ability to rapidly detect variants is important in clinical practice. This study aims to develop a simple polymerase chain reaction (PCR) procedure for detecting NUDT15 variants in Vietnamese patients. Materials and Methods Sanger sequencing was used to determine NUDT15 variants from 200 patients. We designed primers and optimized the PCR procedure for detection of wild-type and variant alleles and compared with Sanger sequencing results. Results The inserted variant c.55_56insGAGTCG was detected by differences in size through conventional PCR. The tetra-primer amplification refractory mutation system PCR was successful in detecting two variations, c.52G > A and c.415C > T. The sensitivity and specificity of PCR procedure achieved 100% when compared to 200 Sanger sequencing results. Conclusion Our PCR procedure is suitable for replacing Sanger sequencing to detect the NUDT15 variants in clinical setting.
Keyphrases
- acute lymphoblastic leukemia
- end stage renal disease
- copy number
- newly diagnosed
- ejection fraction
- chronic kidney disease
- real time pcr
- minimally invasive
- single cell
- prognostic factors
- clinical practice
- peritoneal dialysis
- allogeneic hematopoietic stem cell transplantation
- wild type
- genome wide
- transcription factor
- acute myeloid leukemia
- quantum dots
- sensitive detection