Nanomolar detection of adenosine triphosphate (ATP) using a nanostructured fluorescent chemosensing ensemble.

Ling-Xi HuangQing GuoYong Chen Peter Verwilst Subin SonJia-Bin WuQian-Yong Cao Jong Seung Kim

Published in: Chemical communications (Cambridge, England) (2019)

We report a novel nanostructured chemosensing ensemble PyNp-C13/UD, obtained by self-assembling uranine dye (UD) and an amphiphilic pyridinium salt PyNp-C13. The ensemble was developed for the fluorescence turn-on sensing of ATP in aqueous solutions and inside living cells. The assembly operates via an indicator displacement assay (IDA) method with an ultra-low detection limit of 6.8 nM.

Keyphrases

living cells
fluorescent probe
single molecule
convolutional neural network
label free
loop mediated isothermal amplification
neural network
real time pcr
high throughput
high resolution
quantum dots
protein kinase
single cell
highly efficient