A Simple Determination of Trinitrotoluene (TNT) Based on Fluorescence Quenching of Rhodamine 110 with FRET Mechanism.

Sensitive and selective detection of nitroaromatic explosives is an important issue in regard to human health, environment, public security and military issues. In this study, a simple and sensitive fluorescence quenching - based assay utilizing Rhodamine 110 as fluorophore probe was developed for the determination of trinitrotoluene (TNT). This sensitive fluorometric method could measure the decrease in fluorescence of Rhodamine 110 (λex = 490 nm, λem = 521 nm) owing to the primary amine groups of Rhodamine 110 (different from other rhodamines) capable of donor-acceptor interaction with TNT. The resulting TNT-amine complex can strongly quench the fluorescence emission of Rhodamine 110 by fluorescence resonance energy transfer (FRET) which occurs as the excited Rhodamine 110 fluorophore (donor) transfers its energy to TNT (acceptor) by non-radiative dipole-dipole interaction. Fluorescence quenching varied linearly with TNT concentration, with LOD and the LOQ of 0.71 and 2.38 mg L- 1 TNT, respectively. Similar explosives, common soil ions, and possible camouflage materials were found not to interfere with the proposed method, offering significant advantages with its easy methodology, low-cost, sensitivity, and rapidity of analysis. FRET mechanism based on dye donor-TNT acceptor interaction.