Wide-field time-gated SPAD imager for phasor-based FLIM applications.

Arin Ulku Andrei Ardelean Ivan Michel Antolovic Shimon Weiss Edoardo Charbon Claudio Bruschini Xavier Michalet

Published in: Methods and applications in fluorescence (2020)

We describe the performance of a new wide area time-gated single-photon avalanche diode (SPAD) array for phasor-FLIM, exploring the effect of gate length, gate number and signal intensity on the measured lifetime accuracy and precision. We conclude that the detector functions essentially as an ideal shot noise limited sensor and is capable of video rate FLIM measurement. The phasor approach used in this work appears ideally suited to handle the large amount of data generated by this type of very large sensor (512 × 512 pixels), even in the case of small number of gates and limited photon budget.

Keyphrases

electronic health record
high intensity
air pollution
big data
high throughput
image quality
high density
mass spectrometry
single cell