FGF-21 biomarker detection at the sub-nanogram per mL level in human serum using normal-flow liquid chromatography/tandem mass spectrometry.
Wenqing GaoJiaqian YangRong LiuChuan-Fan DingChengyi XieJiancheng YuKeqi TangPublished in: Rapid communications in mass spectrometry : RCM (2021)
The strategy offered an effective alternative to nano-LC/SRM MS for the quantification of protein biomarkers in a complex biomatrix with much improved reproducibility and operational robustness.
Keyphrases
- liquid chromatography tandem mass spectrometry
- simultaneous determination
- ms ms
- solid phase extraction
- mass spectrometry
- liquid chromatography
- multiple sclerosis
- tandem mass spectrometry
- loop mediated isothermal amplification
- protein protein
- real time pcr
- label free
- amino acid
- binding protein
- gas chromatography
- high resolution mass spectrometry
- quantum dots