A SARS-CoV-2 Spike Ferritin Nanoparticle Vaccine Is Protective and Promotes a Strong Immunological Response in the Cynomolgus Macaque Coronavirus Disease 2019 (COVID-19) Model.
Sara C JohnstonKeersten M RicksInes Lakhal-NaouarAlexandra N JayCaroline SubraJo Lynne RaymondHannah A D KingFranco RossiTamara L ClementsDavid FettererSamantha D TostensonCamila Macedo CincottaHolly R HackCaitlin KuklisSandrine SomanJocelyn KingKristina K PeachmanDohoon KimWei-Hung ChenRajeshwer S SankhalaElizabeth J MartinezAgnes HajduczkiWilliam C ChangMisook ChoePaul V ThomasCaroline E PetersonAlexander AndersonIsabella SwaffordJeffrey R CurrierDominic Paquin-ProulxLinda L JagodzinskiGary R MatyasMangala RaoGregory D GromowskiSheila A PeelLauren WhiteJeffrey M SmithJay W HooperNelson L MichaelKayvon ModjarradM Gordon JoyceAyşegül NalçaDiane L BoltonMargaret L M PittPublished in: Vaccines (2022)
The COVID-19 pandemic has had a staggering impact on social, economic, and public health systems worldwide. Vaccine development and mobilization against SARS-CoV-2 (the etiologic agent of COVID-19) has been rapid. However, novel strategies are still necessary to slow the pandemic, and this includes new approaches to vaccine development and/or delivery that will improve vaccination compliance and demonstrate efficacy against emerging variants. Here, we report on the immunogenicity and efficacy of a SARS-CoV-2 vaccine comprising stabilized, pre-fusion spike protein trimers displayed on a ferritin nanoparticle (SpFN) adjuvanted with either conventional aluminum hydroxide or the Army Liposomal Formulation QS-21 (ALFQ) in a cynomolgus macaque COVID-19 model. Vaccination resulted in robust cell-mediated and humoral responses and a significant reduction in lung lesions following SARS-CoV-2 infection. The strength of the immune response suggests that dose sparing through reduced or single dosing in primates may be possible with this vaccine. Overall, the data support further evaluation of SpFN as a SARS-CoV-2 protein-based vaccine candidate with attention to fractional dosing and schedule optimization.
Keyphrases
- sars cov
- respiratory syndrome coronavirus
- coronavirus disease
- immune response
- healthcare
- mental health
- emergency department
- single cell
- drug delivery
- gold nanoparticles
- copy number
- dna methylation
- mesenchymal stem cells
- cell therapy
- amino acid
- inflammatory response
- protein protein
- bone marrow
- adverse drug
- artificial intelligence