Ring-Size Memory of Galactose-Containing MS/MS Fragments: Application to the Detection of Galactofuranose in Oligosaccharides and Their Sequencing.
Oznur YeniSimon OllivierBaptiste MogeDavid RopartzHélène RogniauxLaurent LegentilVincent FerrieresIsabelle CompagnonPublished in: Journal of the American Chemical Society (2023)
Analysis of glycans remains a difficult task due to their isomeric complexity. Despite recent progress, determining monosaccharide ring size, a type of isomerism, is still challenging due to the high flexibility of the five-membered ring (also called furanose). Galactose is a monosaccharide that can be naturally found in furanose configuration in plant and bacterial polysaccharides. In this study, we used the coupling of tandem mass spectrometry and infrared ion spectroscopy (MS/MS-IR) to investigate compounds containing galactofuranose and galactopyranose. We report the IR fingerprints of monosaccharide fragments and demonstrate for the first time galactose ring-size memory upon collision-induced dissociation (CID) conditions. The linkage of the galactose unit is further obtained by analyzing disaccharide fragments. These findings enable two possible applications. First, labeled oligosaccharide patterns can be analyzed by MS/MS-IR, yielding full sequence information, including the ring size of the galactose unit; second, MS/MS-IR can be readily applied to unlabeled oligosaccharides to rapidly identify the presence of a galactofuranose unit, as a standalone analysis or prior to further sequencing.
Keyphrases
- ms ms
- ultra high performance liquid chromatography
- tandem mass spectrometry
- high performance liquid chromatography
- liquid chromatography tandem mass spectrometry
- high resolution
- simultaneous determination
- gas chromatography
- liquid chromatography
- single cell
- working memory
- solid phase extraction
- healthcare
- room temperature
- genome wide
- computed tomography
- oxidative stress
- gene expression
- men who have sex with men
- drug induced
- label free
- hiv testing
- hiv infected
- amino acid
- real time pcr