A fluorescent probe based on an enhanced ICT effect for Hg 2+ detection and cell imaging.
Shuchen PeiChaozheng LiXinyu PeiXiangyang ZhangYuting ChiWenhong ZengYuanyuan ZhangXiaoling LiaoJun ChenPublished in: Analytical methods : advancing methods and applications (2023)
The mercury ion (Hg 2+ ) has hindered society to some extent due to its high biological toxicity, and a rapid method for Hg 2+ detection is urgently needed. In the present work, two fluorescent probes, YF-Hg and YF-Cl-Hg, were developed. YF-Cl-Hg was produced by introducing an electron-withdrawing substituent (-Cl) into the structure of YF-Hg. The probe YF-Cl-Hg possesses a larger Stokes shift and a more pronounced UV-vis absorption redshift compared to YF-Hg in a pH = 7.4 environment. The reasons for the superior spectral performance of YF-Cl-Hg over YF-Hg were explored by density functional theory (DFT) calculations and UV-vis absorption spectroscopy. Furthermore, the good biocompatibility suggests that YF-Cl-Hg possesses the potential to be a tool for Hg 2+ detection in cells.
Keyphrases
- fluorescent probe
- living cells
- aqueous solution
- density functional theory
- high resolution
- single molecule
- single cell
- optical coherence tomography
- computed tomography
- induced apoptosis
- cell death
- cell proliferation
- magnetic resonance imaging
- mesenchymal stem cells
- signaling pathway
- molecular dynamics simulations
- photodynamic therapy
- molecular docking
- cell cycle arrest
- tissue engineering
- human health
- contrast enhanced