Activation of a Latent Epitope Causing Differential Binding of Antineutrophil Cytoplasmic Antibodies to Proteinase 3.
Marta Casal MouraGwen E ThompsonDarlene R NelsonLynn A FussnerAmber M HummelDieter E JenneDaniel EmerlingFernando C FervenzaCees G M KallenbergCarol A LangfordW Joseph McCunePeter A MerkelPaul A MonachPhilip SeoRobert F SpieraE William St ClairSteven R YtterbergJohn H StoneWilliam H RobinsonUlrich Specksnull nullPublished in: Arthritis & rheumatology (Hoboken, N.J.) (2022)
The preferential binding of PR3-ANCAs from patients, such as the selective binding of moANCA518 to iHm5, is conferred by increased antigenicity of epitope 3 on iHm5. This can also be induced on iPR3 when captured by monoclonal antibody MCPR2. This previously unrecognized characteristic of PR3-ANCA interactions with its target antigen has implications for studying antibody-mediated autoimmune diseases, understanding variable performance characteristics of immunoassays, and design of potential novel treatment approaches.