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Bioinspired Fluorine Labeling for 19 F NMR-Based Plasma Amine Profiling.

Quynh Huong DuongEun-Jeong KwahkJumi KimHahyoun ParkHeyjin ChoHyunwoo Kim
Published in: Analytical chemistry (2024)
Metabolite profiling serves as a powerful tool that advances our understanding of biological systems, disease mechanisms, and environmental interactions. In this study, we present an approach employing 19 F-nuclear magnetic resonance ( 19 F NMR) spectroscopy for plasma amine profiling. This method utilizes a highly efficient and reliable fluorine-labeling reagent, 3,5-difluorosalicylaldehyde, which effectively emulates pyridoxal phosphate, facilitating the formation of Schiff base compounds with primary amines. The fluorine labeling allows for distinct resolution of 19 F NMR signals from amine mixtures, leading to precise identification and quantification of amine metabolites in human plasma. This advancement offers valuable tools for furthering metabolomics research.
Keyphrases
  • magnetic resonance
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  • positron emission tomography
  • single cell
  • pet imaging
  • high resolution
  • mass spectrometry
  • contrast enhanced
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  • magnetic resonance imaging