Analytical validation of an automated assay for the measurement of adenosine deaminase (ADA) and its isoenzymes in saliva and a pilot evaluation of their changes in patients with SARS-CoV-2 infection.

Lorena Franco-MartínezFernando TeclesAlberto Torres-CanteroEnrique BernalIndra San LázaroMaría José AlcarazMaría R Vicente-RomeroElsa LamyCristina Sánchez-ResaltCamila P RubioAsta TvarijonaviciuteSilvia Martinez-SubielaJosé J Cerón

Published in: Clinical chemistry and laboratory medicine (2021)

tADA and its isoenzymes ADA1 and ADA2 can be measured accurately and precisely in saliva samples in a rapid, economical, and reproducible way and can be analyzed after chemical inactivation with Triton X-100 and NP-40. Besides, the changes observed in tADA and isoenzymes in individuals with COVID-19 open the possibility of their potential use as non-invasive biomarkers in this disease.

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