Non-glycosidic iridoids from the roots of Patrinia scabra and their nitric oxide production inhibitory effects.
Da Hye LeeJi-Sun ShinJin Su LeeShin-Young KangHee-Soo HanSeung Mok RyuKyung-Tae LeeDongho LeeDae-Sik JangPublished in: Archives of pharmacal research (2019)
Phytochemical investigation on the 70% aqueous EtOH extract from the roots of Patrinia scabra led to the isolation and characterization of five new non-glycosidic iridoids, patriscabrins F-J (1-5), along with a known iridoid 11-ethoxyviburtinal (6). The structures of the new compounds 1-5 were determined by interpretation of spectroscopic data, particularly by 1D- and 2D-NMR and ECD studies. Thereafter, the isolates 1-6 were evaluated for their inhibitory effects on lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells. Of these, patriscabrin F (1) exhibited the most potent inhibitory effect with observed IC50 value of 14.1 μM. In addition, patriscabrin G (2) and 11-ethoxyviburtinal (6) showed IC50 values 24.6 and 35.5 μM, respectively.
Keyphrases
- nitric oxide
- lipopolysaccharide induced
- inflammatory response
- induced apoptosis
- nitric oxide synthase
- high resolution
- hydrogen peroxide
- cell cycle arrest
- molecular docking
- oxidative stress
- magnetic resonance
- anti inflammatory
- electronic health record
- big data
- endoplasmic reticulum stress
- cell death
- mass spectrometry
- signaling pathway
- cell proliferation