Determination of sugar content in honey using LC-Raman and programmable pump-Raman methods.
Liang-Hung WengHirotsugu HiramatsuPublished in: Analytical methods : advancing methods and applications (2023)
We combined (i) liquid chromatography and Raman spectrometry (LC-Raman) and (ii) programmable pump and Raman spectrometry (PP-Raman) to separate and identify compounds in a mixture. These techniques were applied to conduct a quantitative analysis of the sugars in honey. The spectral and temporal axes of the LC-Raman data were analyzed using the MCR-ALS analysis procedure, which enabled the separation and identification of four sugars (glucose, fructose, sucrose, and trehalose). The PP-Raman method was employed to examine the sugar concentration dependence of the intensity pattern of the Raman spectrum, and the linear concentration dependence of the intensity was obtained. The sugar contents were quantitatively determined from the integrated area of the elution peaks. The result was consistent with those derived from mass spectrometry and previous studies. The origin of the errors in the derived sugar contents is discussed. Our study presents a novel quantitative LC-Raman spectrometric method that does not rely on resonance or surface enhancement effects.
Keyphrases
- liquid chromatography
- mass spectrometry
- raman spectroscopy
- solid phase extraction
- label free
- simultaneous determination
- high resolution mass spectrometry
- tandem mass spectrometry
- high resolution
- high performance liquid chromatography
- high intensity
- type diabetes
- magnetic resonance
- patient safety
- magnetic resonance imaging
- gas chromatography
- ms ms
- optical coherence tomography
- artificial intelligence
- adipose tissue
- insulin resistance
- quality improvement
- capillary electrophoresis
- case control