Impact of Hyaluronic Acid on the Viability of Mesenchymal Cells Derived from Adipose Tissue Grown in Collagen Type I/III Membrane.
Camila Cohen KalekaPedro DebieuxEliane AntonioliEder ZucconiMoisés CohenMário FerrettiPublished in: Revista brasileira de ortopedia (2022)
Objective To evaluate in vitro the viability of mesenchymal stem cells derived from adipose tissue (AD-MSCs) in different commercial solutions of hyaluronic acid (HA) before and after being sowed in collagen I/III membrane. Methods In the first stage, the interaction between AD-MSCs was analyzed with seven different commercial products of HA, phosphate buffered saline (PBS), and bovine fetal serum (BFS), performed by counting living and dead cells after 24, 48 and 72 hours. Five products with a higher number of living cells were selected and the interaction between HA with AD-MSCs and type I/III collagen membrane was evaluated by counting living and dead cells in the same time interval (24, 48 and 72 hours). Results In both situations analyzed (HA + AD-MSCs and HA + AD-MSCs + membrane), BFS presented the highest percentage of living cells after 24, 48 and 72 hours, a result higher than that of HA. Conclusion The association of HA with AD-MSCs, with or without membrane, showed no superiority in cell viability when compared with BFS.
Keyphrases
- mesenchymal stem cells
- living cells
- hyaluronic acid
- umbilical cord
- induced apoptosis
- adipose tissue
- fluorescent probe
- cell cycle arrest
- bone marrow
- single molecule
- endoplasmic reticulum stress
- insulin resistance
- stem cells
- oxidative stress
- signaling pathway
- high fat diet
- cell therapy
- type diabetes
- cell proliferation
- tissue engineering
- plant growth