The Emerging Challenge of Diagnosing Drug-resistant Tubercular Uveitis: Experience of 110 Eyes from North India.
Kusum SharmaAmod GuptaMegha SharmaAman SharmaReema BansalSurya Prakash SharmaRaman Deep SinghVishali GuptaPublished in: Ocular immunology and inflammation (2019)
Background: Rapid and timely diagnosis of tubercular uveitis (TBU) is of paramount importance to save these eyes from blindness. The present study was, therefore, undertaken to carry out a comparative evaluation of Gene Xpert MTB/RIF (Xpert), MTBDRplus and Multiplex PCR (MPCR) for the diagnosis of TBU. These tests were performed on vitreous fluid of 110 patients with presumed TBU and 90 controls. rpoB gene sequencing confirmed Rifampicin resistance.Results: Xpert, MTBDRplus and MPCR were positive in 19(17.2%),38 (34.5%) and 79 (71.8 %) patients, respectively. All tests were negative in all controls. Rif resistance was detected in 3 by Xpert and 7 by MTBDRplus. MPCR followed by rpoB gene sequencing detected Rif resistance in 6 cases. One case of false Rif resistance was reported each by MTBDRplus and Xpert.Conclusion: MPCR followed by rpoB sequencing is a robust technique for the diagnosis of paucibacilliary condition like TBU and reliable detection of drug resistance.
Keyphrases
- pulmonary tuberculosis
- drug resistant
- mycobacterium tuberculosis
- single cell
- genome wide
- multidrug resistant
- copy number
- end stage renal disease
- acinetobacter baumannii
- juvenile idiopathic arthritis
- ejection fraction
- newly diagnosed
- chronic kidney disease
- genome wide identification
- gene expression
- prognostic factors
- high throughput
- systemic lupus erythematosus
- tertiary care
- disease activity