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A practical, low-cost, short-term storage method for genomic DNA.

Demir Özdemir
Published in: BioTechniques (2021)
The aim of this study was to assess the DNA preservation capability of cellulose paper towel and blotting paper as low-cost alternatives to commercial DNA preservation products. Chicken blood was applied as DNA source to each paper towel, blotting paper, FTA® cards and DNA/RNA Shield™. All samples were stored at room temperature for 130 days. DNA extraction from dried blood spots was performed after various time periods using Tris-EDTA and NaOH protocols. PCR activity and the mean amount of DNA isolated from paper towels were reliable. The results of this study demonstrated that cellulose-based blotting paper and especially paper towel had considerable DNA binding and preservation capacity for at least 130 days at room temperature without DNA degradation.
Keyphrases
  • circulating tumor
  • room temperature
  • cell free
  • single molecule
  • low cost
  • nucleic acid
  • ionic liquid
  • dna binding
  • circulating tumor cells
  • gene expression