Facile synthesis of biogenic silica nanomaterial loaded transparent tragacanth gum hydrogels with improved physicochemical properties and inherent anti-bacterial activity.
Mohini VermaAqib Iqbal DarAmitabha AcharyaPublished in: Nanoscale (2022)
In this report, biogenic, crystalline (∼60.5 ± 2%) bowknot structured silica nanoparticles (BSNPs) of length ∼ 274 ± 7 nm and width ∼ 36 ± 2 nm were isolated from invasive species viz . Lantana camara . These were then chemically modified using nitrogen containing moieties viz . APTES and CTAB. These modified BSNPs were then used as electrostatic cross-linking agents for the formation of tragacanth gum (TG) hydrogels. The cytocompatible CTAB@BSNP-TG hydrogels documented ∼10-12 fold enhancement in anti-bacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa when compared with TG hydrogels. Disruption of the bacterial membrane by ROS generation and protein leakage were responsible for anti-bacterial activity. A cell migration assay suggested that CTAB@BSNP-TG augmented the cell proliferation of NIH-3T3 cells compared to other TG hydrogels. The present study will pave the path for the development of organic-inorganic hybrid nanocomposite-based hydrogels for anti-bacterial and cell migration applications.
Keyphrases
- cell migration
- drug delivery
- hyaluronic acid
- drug release
- extracellular matrix
- wound healing
- tissue engineering
- pseudomonas aeruginosa
- staphylococcus aureus
- cell proliferation
- photodynamic therapy
- dna damage
- cell death
- cancer therapy
- cystic fibrosis
- escherichia coli
- high throughput
- biofilm formation
- drug resistant
- acinetobacter baumannii
- mass spectrometry
- reactive oxygen species
- oxidative stress
- gold nanoparticles
- ionic liquid
- amino acid
- room temperature
- molecular dynamics simulations
- light emitting