Automated, Transferable, and Ethanol-Free Radiosynthesis of [ 11 C]Butanol.
Olujide OyeniranLinshan LiuConfidence RaymondPaulien MoyaertMichael S KovacsUdunna C AnazodoJustin W HicksPublished in: ACS chemical neuroscience (2024)
Cerebral blood flow and blood-brain barrier permeability assessment are crucial hemodynamic parameters to measure under neurological conditions. In conjunction with positron emission tomography (PET), oxygen-15-labeled water has emerged as a gold standard for measuring cerebral perfusion; however, at higher flow rates, [ 15 O]water extraction becomes nonlinear. In such a scenario, freely diffusible [ 11 C]butanol can provide a truer estimate. Radiosyntheses of [ 11 C]butanol reported to date are protracted, are not automated, or require ethanol in the final formulation. By using a flow-based, captive solvent approach on a commercially available radiosynthesizer, we automated and reduced the synthesis time to 28 min. Forgoing cartridge-based purification for an aqueous high-performance liquid chromatography method, we obtained high purity [ 11 C]butanol in ethanol-free phosphate buffered saline in sufficient yields for clinical PET studies. We here report our expedited, automated, and ethanol-free radiosynthesis of [ 11 C]butanol along with preliminary imaging of a porcine subject.
Keyphrases
- pet imaging
- positron emission tomography
- blood brain barrier
- computed tomography
- deep learning
- machine learning
- high performance liquid chromatography
- high throughput
- cerebral blood flow
- pet ct
- cerebral ischemia
- tandem mass spectrometry
- high resolution
- mass spectrometry
- solid phase extraction
- ionic liquid
- drug delivery
- simultaneous determination
- magnetic resonance imaging
- brain injury
- ms ms
- magnetic resonance
- liquid chromatography