Development and validation of a novel 7α-hydroxy-4-cholesten-3-one (C4) liquid chromatography tandem mass spectrometry method and its utility to assess pre-analytical stability.
Jonathan Shaun AtkinsBrian G KeevilAngela E TaylorChristian LudwigJames M HawleyPublished in: Clinical chemistry and laboratory medicine (2024)
SeHCAT for BAD investigation. C4 was found to decline in unseparated blood over time; however, after 12 h the mean change was <5 % from baseline. Our results suggest C4 is suitable for collection from both primary and secondary care prior to gastroenterology referral.